| 序号 | A 温度(℃) | B pH | C 底物浓度(%) | D 酶浓度(%) | DPPH 自由基清除率(%) |
| 1 | 1 (50℃) | 1 (8.5) | 1 (0.8%) | 1 (1.3%) | 82.21 |
| 2 | 1 | 2 (9.0) | 2 (1.0%) | 2 (1.5%) | 86.60 |
| 3 | 1 | 3 (9.5) | 3 (1.2%) | 3 (1.7%) | 32.04 |
| 4 | 2 (55℃) | 1 | 2 | 3 | 60.97 |
| 5 | 2 | 2 | 3 | 1 | 34.15 |
| 6 | 2 | 3 | 1 | 2 | 82.67 |
| 7 | 3 (60℃) | 1 | 3 | 2 | 76.21 |
| 8 | 3 | 2 | 1 | 3 | 58.66 |
| 9 | 3 | 3 | 2 | 1 | 88.45 |
| K1 | 200.85 | 219.39 | 223.54 | 204.81 | T = 643.78 |
| K2 | 177.79 | 179.41 | 236.02 | 245.48 | |
| K3 | 223.32 | 203.16 | 142.40 | 151.67 | |
| k1 | 66.95 | 73.13 | 74.51 | 68.27 | |
| k2 | 59.26 | 59.80 | 78.67 | 81.83 | |
| k3 | 74.44 | 67.72 | 47.47 | 50.56 | |
| R | 15.18 | 13.33 | 31.20 | 31.27 | |
| 因素主→次 | DCAB |
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| 最优组合条件 | D1C2A3B3 |
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