60 mL batch culture

200 mL fed-batch culture

3 L fed-batch culture

Culture time (d)

6.00

8.00

7.00

Max cell density (cells/mL)

5.60

5.82

5.00

Collecting the supernatants (L)

0.06

0.22

3.30

Protein concentration (mg/L)a

10.00

18.30

20.50

Protein purity (%)b

95.00