| 管号 | 1 | 2 | 3 | 4 | 5 | 6 |
| 100 μg/mL标准蛋白溶液(ml) | 0 | 0.2 | 0.4 | 0.6 | 0.8 | 1 |
| 蒸馏水(ml) | 1.0 | 0.8 | 0.6 | 0.4 | 0.2 | 0 |
| 考马斯亮蓝G-250试剂(ml) | 5.0 | 5.0 | 5.0 | 5.0 | 5.0 | 5.0 |
| 蛋白质含量(μg) | 0 | 20 | 40 | 60 | 80 | 100 |