| 试剂 | 体积 |
| 5 × FastPfu Buffer | 4 μL |
| 2.5 mM dNTPs | 2 μL |
| Forward Primer (5 μM) | 0.8 μL |
| Reverse Primer (5 μM) | 0.8 μL |
| FastPfu Polymerase | 0.4 μL |
| BSA | 0.2 μL |
| Template DNA | 10 ng |
| ddH2O | Up to 20 μL |