| AA residue | DNA/RNA | DNA/RNA chain | control AA residue |
| 62 T | 63URA | sgRNA | 61A |
| 76 K | 49ADE | sgRNA | 75R |
| 163 K | 48ADE | sgRNA | 164F |
| 401 K | 45URA | sgRNA | 400R |
| 839 D | 12THY | targetDNA | 840H |
| 866 K | 21THY | targetDNA | 864R |
| 1019R | 28THY | targetDNA | 1020K |