引物序号与代号 Primer No. | 序列 Sequence (5'-3') | 退火温度(℃) Anneal temperature (˚C) | |
1 | 814 | CTCTCTCTCTCTCTCTA | 50 |
2 | 816 | CACACACACACACACAT | 52 |
3 | 829 | TGTGTGTGTGTGTGTGC | 51 |
4 | 836 | AGAGAGAGAGAGAGAGCTA | 52 |
5 | 840 | GAGAGAGAGAGAGAGACTA | 52 |
6 | 851 | GTGTGTGTGTGTGTGTCTG | 52 |
7 | 855 | ACACACACACACACACCTT | 54 |
8 | 857 | ACACACACACACACACCTG | 50 |
9 | 886 | ACGAGTACGCTCTCTCTCTCTCT | 50 |
10 | 887 | AGTACGAGTCTCTCTCTCTCTCT | 50 |
11 | 889 | AGTCGTAGTACACACACACACAC | 51 |
12 | 890 | ACGAGTACGGTGTGTGTGTGTGT | 53 |