| Validation parameter | rat plasma | PBS | ||
| A-YM0402 | B-YM0402 | A-YM0402 | B-YM0402 | |
| Intra-repeatability (RSD%) |
|
| ||
| Low | 9.1 | 8.4 | 8.6 | 7.9 |
| Medium | 8.7 | 7.1 | 5.5 | 7.1 |
| High | 6.2 | 6.5 | 4.2 | 5.7 |
| Inter-repeatability (RSD%) |
|
| ||
| Low | 2.1 | 3.3 | 6.9 | 5.0 |
| Medium | 2.1 | 0.8 | 4.3 | 6.4 |
| High | 1.1 | 3.1 | 1.4 | 3.8 |
| Accuracy (%) |
|
| ||
| Low | 98.3 (7.3) | 102.0 (8.3) | 99.2 (3.8) | 100.8 (4.4) |
| Medium | 100.6 (2.3) | 101.0 (7.2) | 103.2 (6.3) | 98.7 (6.9) |
| High | 98.7 (4.9) | 100.7 (5.9) | 105.7 (6.5) | 101.6 (8.4) |
| LOQ (μM) | 0.1 | 0.1 | 0.03 | 0.03 |
| Linear range (μM) | 0.1~200 | 0.1~200 | 0.03~200 | 0.03~200 |
| y-Intercept (S.D.) | 0.416 (0.084) | 0.501 (0.059) | 0.088 (0.006) | 0.019 (0.003) |
| Slope (S.D.) | 0.167 (0.083) | 0.163 (0.001) | 0.509 (0.009) | 0.102 (0.002) |
| r | 0.999 | 0.999 | 0.99 | 0.99 |