| Primer | Sequence(5’-3’) | Amplicon length (bp) | Optimum Tm (˚C) |
| LGTase1 | 5’ CGAGAATAATGGGAACTG 3’ | 1600 bp | 46 |
| LGTase2 | 5’TGAAAGGGAATAGATAAGTAC 3’ | ||
| LGTF2 | 5’ACTCGTAGCATCGGGGGTGCCGGT 3’ | 208 bp | 52 |
| LGTA1 | 5’ TCAGCGCGTTCTCCTCCAGCGCCA 3’ | ||
| LGTF2 | 5’ACTCGTAGCATCGGGGGTGCCGGT 3’ | 208 bp | 52 |
| LGTA2 | 5’ TCAGCGCGTCCTTCA GCTCCG 3’ |