| 1xTaq PCR Mix | 46 uL | 95℃ | 5 min | 1 cycle |
| gDNA | 2 uL | 95℃ | 30 s | 35 cycles |
| ITS1 | 1 uL | 55℃ | 30 s | |
| ITS4 | 1 uL | 72℃ | 60 s | |
|
|
| 72℃ | 5 min | 1 cycle |