| 样品 Sample | 添加量 Addition amount |
| 10 × PCR Buffer | 5 μl |
| dNTP Mixture | 1 μl |
| Primer 1 | 1 μl |
| Primer 2 | 1 μl |
| Template DNA | 2 μl |
| Ex-Taq | 1 μl |
| Sterile water | up to 50 μl |