| 限制性核酸内切酶 | 识别序列 | SNP位点/突变 | 碱基改变数量 |
| AarI | CACCTGCN4^ | TGAAG C AGGTG | 1 |
| AcvI | CAC^GTG | TGAAG C ACGTG | 1 |
| BisI | GC^NGC | TGCAG C AAGTG | 1 |
| BlsI | GCN^GC | TGCAG C AAGTG | 1 |
| BspMI | ACCTGCN4^ | TGAAG C AGGTG | 1 |
| BstBAI | YAC^GTR | TGAAG C ACGTG | 1 |
| BstC8I | GCN^NGC | TGAAG C AAGCG | 1 |
| BstNSI | RCATG^Y | TGAAG C ATGTG | 1 |
| BstV1I | GCAGCN8^ | TGCAG C AAGTG | 1 |
| CviAII | C^ATG | TGAAG C ATGTG | 1 |
| FaeI | CATG^ | TGAAG C ATGTG | 1 |
| FaiI | YA^TR | TGAAG C ATGTG | 1 |
| FatI | ^CATG | TGAAG C ATGTG | 1 |
| HgaI | GACGCN5^ | TGACG C AAGTG | 1 |
| Hpy188III | TC^N2GA | TCAAG A AAGTG | 1 |
| HpyAV | CCTTCN6^ | TGAAG A AGGTG | 1 |
| HpyCH4V | TG^CA | TGATG C AAGTG | 1 |
| LpnPI | CCDGN10^ | TGAAG C AGGTG | 1 |
| MboII | GAAGAN8^ | TGAAG A AAGTG | 0 |
| MspJI | CN2RN9^ | TGAAG C AAGTG | 0 |
| MunI | C^AATTG | TGAAG C AATTG | 1 |
| SfaNI | GCATCN5^ | TGATG C AAGTG | 1 |
| SgeI | CN2GN9^ | TGAAG C AAGTG | 0 |
| TseI | G^CWGC | TGCAG C AAGTG | 1 |
| TspDTI | ATGAAN11^ | TGATG A AAGTG | 1 |