Components

Concentration

Vdh

2 μM

Substrate molecules

10 mM

NADPH

200 μM

GDH

1 U

Tris·HCl, pH 7.5

50 mM

NaCl

100 mM

PMβCD

0.05%, w/v

Fdx

100 μg/mL

Fdr

0.1 U/mL