| 耐药元件种类 | 基因 | 序列(5’-3’) | 片段大小/bp | 退火温度/℃ |
| 整合子 | I类整合子 | F：ACGAGCGCAAGGTTTCGGT | 565 | 52 |
| R：GAAAGGTCTGGTCATACATG | ||||
| β-内酰胺类 | blaSHV | F：AGCCGCTTGAGCAAATTAAAC | 786 | 55 |
| R：GTTGCCAGTGCTCGATCAGC | ||||
| blaTEM | F：CATTTCCGTGTCGCCCTTATTC | 846 | 55 | |
| R：CCAATGCTTAATCAGTGAGGC | ||||
| blaCTXM-1 | F：CGTCACGCTGTTGTTAGGAA | 781 | 55 | |
| R：ACGGCTTTCTGCCTTAGGTT | ||||
| blaCTXM-2 | F：CTCAGAGCATTCGCCGCTCA | 843 | 55 | |
| R：CCGCCGCAGCCAGAATATCC | ||||
| blaCTXM-9 | F：GCGCATGGTGACAAAGAGAGTGCAA | 876 | 55 | |
| R：GTTACAGCCCTTCGGCGATGATTC | ||||
| blaCTXM-8/25 | F：CCAGGCGAACGATGTTCAACA | 730 | 55 | |
| R：CGGCTCCGACTGGGTGAAGTA | ||||
| blaNDM | F：GGTTTGGCGATCTGGTTTTC | 621 | 55 | |
| R：CGGAATGGCTCATCACGATC | ||||
| 多粘菌素类 | CLR5 | F：CGGTCAGTCCGTTTGTTC | 309 | 55 |
| R：CTTGGTCGGTCTGTA GGG | ||||
| 四环素类 | tetA | F：GCTACATCCTGCTTGCCTTC | 210 | 54.6 |
| R：CATAGATCGCCGTGAAGAGG | ||||
| tetB | F：GGTTGAGACGCAATCGAATT | 206 | 52.9 | |
| R：AGGCTTGGAATACTGAGTGTAA | ||||
| 磺胺类 | sul1 | F：CGCACCGGAAACATCGCTGCAC | 163 | 55.8 |
| R：TGAAGTTCCGCCGCAAGGCTCG | ||||
| sul2 | F：GCACTCCAGCAGGCTCGTAA | 191 | 60 | |
| R：CGGGAATGCCATCTGCCTTGAG | ||||
| sulA | F：GCACTCCAGCAGGCTCGTAA | 198 | 56.8 | |
| R：CTCTGCCACCTGACTTTTCCA | ||||
| 质粒 | IncB/O | F：GCGGTCCGGAAAGCCAGAAAAC | 159 | 60 |
| R：TCTGCGTTCCGCCAAGTTCGA | ||||
| IncFIC | F：GTGAACTGGCAGATGAGGAAGG | 262 | 60 | |
| R：TTCTCCTCGTCGCCAAACTAGAT | ||||
| IncP | F：CTATGGCCCTGCAAACGCGCCAGAAA | 534 | 60 | |
| R：TCACGCGCCAGGGCGCAGCC | ||||
| IncFIIA | F：CTGTCGTAAGCTGATGGC | 270 | 60 | |
| R：CTCTGCCACAAACTTCAGC | ||||
| IncFIA | F：CCATGCTGGTTCTAGAGAAGGTG | 462 | 60 | |
| R：GTATATCCTTACTGGCTTCCGCAG | ||||
| IncFIB | F：GGAGTTCTGACACACGATTTTCTG | 702 | 60 | |
| R：CTCCCGTCGCTTCAGGGCATT | ||||
| IncI1 | F：CGAAAGCCGGACGGCAGAA | 139 | 60 | |
| R：TCGTCGTTCCGCCAAGTTCGT | ||||
| IncFrep | F：TGATCGTTTAAGGAATTTTG | 270 | 60 | |
| R：GAAGATCAGTCACACCATCC | ||||
| IncN | F：GTCTAACGAGCTTACCGAAG | 559 | 60 | |
| R：GTTTCAACTCTGCCAAGTTC | ||||
| IncL/M | F：GGATGAAAACTATCAGCATCTGAAG | 785 | 60 | |
| R：CTGCAGGGGCGATTCTTTAGG |