菌株或质粒

特性

来源

菌株

E. coli BL21

E. coli B F⁻ omp T gal dcm lon hsdSB (rB⁻mB⁻)

本实验室保藏

E. coli BL21 Δalr-Red

derivative of BL21, alr deletion using λ-Red

本研究构建

E. coli BL21 Δalr-CRIPSR

derivative of BL21, alr deletion using CRISPR/Cas9

本研究构建

质粒

pKD46

oriR101 Rep101(Ts) araC-P_BAD Amp^r

Datsenko and Wanner [12]

pKD13

Kan^r FRT

Datsenko and Wanner [12]

pCP20

TS-rep FLT Cm^r Amp^r

Datsenko and Wanner [12]

pCas

Rep101(Ts) P_cas-Cas9 P_araB-Red P_trc-sgRNA-pMB1

Jiang et al. [13]

pTargetF

pMB1 aadA sgRNA

Jiang et al. [13]

pTargetF-alr

pMB1 aadA sgRNA-alr

本研究构建