| 引物名称 | 引物序列(5′-3′) |
| alr-sgRNA | CTGTTCTTCGTTATGCACGG |
| alr-N20-F | CTGTTCTTCGTTATGCACGGGTTTTAGAGCTAGAAATAGC |
| alr-N20-R | CCGTGCATAACGAAGAACAGACTAGTATTATACCTAGGAC |
| alr-U-F | TCGACTACCTGCAACTGATG |
| alr-U-R | CATCGCGACCCTTGAAGTCAACGAAGACGTTGCAGGTTGT |
| alr-D-F | ACAACCTGCAACGTCTTCGATGACTTCAAGGGTCGCGATG |
| alr-D-R | ATGGCGATAGCAGTTAAGCC |
| alr-JC-F | GTCGCGCTTCGACAACTATG |
| alr-JC-R | GTTCTCCAGGTTTACGGGCA |
| P1 | ATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGC GACACAAGTGTAGGCTGGAGCTGCTTC |
| P2 | TTAATCCACGTATTTCATCGCGACCCTTGAAGTCAGGCGC GTAATAACTGTCAAACATGAGAATTAATTCCG |
| P3 | GCGTGAATCTGGCTCTATCG |
| P4 | GCGTAAGAATCGGGTCGCCA |
| P5 | CTATATCGATGACTCCTCCGGC |
| P6 | CTCCAGCCTACACTAATCACAACAGTTGCCGCTTG |
| P7 | GTGATTAGTGTAGGCTGGAGCTGCTTCG |
| P8 | CCACGTATTTCATCTGTCAAACATGAGAATTAATTCCG |
| P9 | TTGACAGATGAAATACGTGGATTAATCGTTCTG |
| P10 | GGTCCGCACCAAACAGCAGC |