| 试剂名称 | 体积 | ||
|
| 长片段 | 短片段 | 阴性对照 |
| 2×phanta buffer | 10 μL | 10 μL | 10 μL |
| dNTPs | 0.4 μL | 0.4 μL | 0.4 μL |
| CCL25bF2b | 1 μL | 1 μL | 1 μL |
| CCL25bR2U | 1 μL | 1 μL | - |
| UPL | 1 μL | - | 1 μL |
| 模板DNA | 1 μL | 1 μL | 1 μL |
| Phanta酶 | 0.4 μL | 0.4 μL | 0.4 μL |
| ddH2O | up to 20 μL | up to 20 μL | up to 20 μL |