重复单元 | 引物名称 | 序列(5'~3') | 长度(bp) | 退火温度(℃) |
(TTAT)5 | G2-F | AAAGATTGGGTTTCGTGGTG | 20 | 57.5 |
G2-R | CCGTATCAGGGTATTTCAACTT | 22 | ||
(AG)9 | G3-F | CCTTGCTTGACTTGCCTCT | 19 | 57.5 |
G3-R | CTTACCCTCAACCCAACATTA | 21 | ||
(AT)10 | G4-F | TCACCACCTTCTGGCTCATCA | 21 | 57 |
G4-R | ACTCGGTTCATCCCTTGATTTTG | 23 | ||
(AAAT)5 | G5-F | AGAGGCATCTAAGCCACAACAG | 22 | 58.5 |
G5-R | GTTTGGATTCAGGGTGGACAT | 21 | ||
(TTTTAT)5 | G6-F | AGCGACGGCGAGAAACTA | 18 | 58 |
G6-R | CTTTTGCCCACTTATGTCCC | 20 | ||
(TC)11 | G7-F | GGGAAGGTAGCGGGAATG | 18 | 59 |
G7-R | GGCA ATACGGAGATGGTGTC | 20 | ||
(AG)14 | G8-F | GATTCAAGCCTCCGAACCTG | 20 | 59 |
G8-R | CGATGGCAAACAAACTCAAAG | 21 | ||
(TC)17 | G10-F | GGTCCACTATTCAACACCAACA | 22 | 57.5 |
G10-R | CTTGAAGCCCAACTGACCC | 19 |