序号 No.

解离液 Isolation buffer

组分Component

1

Li

100 mmol/L citric acid; 0.2% Triton X-100; pH 2.0~3.0

2

OTTO1

OTTOA:100 mmol/L citric acid; 0.5% Tween 20; 20 mmol/L β-mercaptoethanol; pH 2~3

OTTOB: 400 mmol/L Na₂HPO4·12H₂O; 20 mmol/L β-mercaptoethanol; pH 8~9

3

Pfosser

200 mmol/L Tris; 4 mmol/L MgCl₂ ·6H₂O; 0.5% Triton X-100; 20 mmol /L β-mercaptoethanol; pH 7.5

4

Galbraith

45 mmol/L MgCl₂, 30 mmol/L Sodium citrate, 20 mmol/L MOPS, 0.1% (V/V) Triton X-100, pH 7.0

5

Hepes

10 mmol/L MgSO₄·7H₂O, 50 mmol/L KCl, 5 mmol/L Hepes (pH 8), 6.5 mmol/L DTT, 0.25% Triton X-100

6

WPB

0.2 mmol/L Tris·HCl, 4 mmol/L MgCl₂·6H₂O, 2 mmol/L Na₂EDTA·2H₂O, 86 mmol/L NaCl, 10 mmol/L Na₂S₂O₅, 1% PVP-10, 1% Triton X-100, pH 7.5

7

LBO1

15 mmol/L Tris, 2 mmol/L Na₂EDTA, 0.5 mmol/L Spermine·4HCl, 80 mmol/L KCl, 20 mmol/L NaCl, 0.1% (V/V) Triton X-100, 15 mmol/L β-巯基乙醇, pH8.0

8

MGB

45 mmol/L MgCl₂, 20 mmol/L MOPS, 30 mmol/L Sodium citrate, 1% (W/V) PVP-40, 0.2% (V/V) Triton X-100, 10 mmol/L Na₂EDTA, 20 mmol/L β-巯基乙醇, pH 7.0

9

GPB

0.5 mmol/L Spermine·4HCl, 30 mmol/L Sodium citrate, 20 mmol/L MOPS, 80 mmol/L KCl, 20 mmol/L NaCl, 0.5% (V/V) Triton X-100, pH 7.0