Gene

Upstream primer

Downstream primer

miR-135a-3p

5'-TGCGGTGTAGGGATGGAAGCCAT-3

5'-CCAGTGCAGGGTCCGAGGT -3'

GAPDH

5'-AAGAAGGTGGTGAAGCAGGC-3'

5'-TCCACCACCCTGTTGCTGTA-3'

U6

5'-CTCGCTTCGGCAGCACA -3'

5'-AACGCTTCACGAATTTGCGT -3'