引物名称	引物序列	PCR产物大小
Trim26-FRT-tF1	GCCTGGCAGTGTCTAGCCAG	FL: 660 bp wt: 510 bp
Trim26-FRT-tR1	ATTGATACCCGCTCGATCCT	FL: 660 bp wt: 510 bp
Trim26-LoxP-tF1	ACACCAAGGACTTCGCCAACAA	Fln = fl = 513 bp wt: 390 bp
Trim26-LoxP-tR1	TGACAATTACAAGGCAAGCA	Fln = fl = 513 bp wt: 390 bp
Neo-5R	GGCTGGACGTAAACTCCTC	Fln: 414 bp
Trim26-FRT-tR1	ATTGATACCCGCTCGATCCT	Fln: 414 bp
Flp-F2	GACAAGCGTTAGTAGGCACATATAC	T = 325 bp
Flp-R2	GCTCCAATTTCCCACAACATTAGT	T = 325 bp
Myo-F1	TTACGTCCATCGTGGACAGC	245 bp
Myo-R1	TGGGCTGGGTGTTAGCCTTA	245 bp