| 引物 | 产物长度 size | 引物长度(bp) bp | Tm (˚C) | “C”s | 引物序列 |
| F-N-RAGE | 106 | 25 | 59 | - | CCCCATGCATCCCCACCCCCGC |
| R-N-RAGE | 25 | 57 | - | ATCTGAGTCAGACACTCTCGTC | |
| F-M-RAGE | 112 | 25 | 55 | 15 | CGTTTTTATGTATTTTTATTTTCGT |
| R-M-RAGE | 25 | 55 | 4 | AATATCTAAATCAAACACTCTCGTC | |
| F-U-RAGE | 112 | 25 | 52 | 15 | TGTTTTTATGTATTTTTATTTTTGT |
| R-U-RAGE | 25 | 53 | 4 | AATATCTAAATCAAACACTCTCATC |