| 试剂加入 | 10%分离胶 | 5%浓缩胶 |
| 蒸馏水 | 2 ml | 1.4 ml |
| 30% Acr-Bis (29:1) | 1.65 ml | 0.33 ml |
| Tris-HCI (PH = 8.0) 1.5 M | 1.25 ml | / |
| Tris-HCI (PH = 6.8) 1.0 M | / | 0.25 ml |
| 10% SDS | 0.05 ml | 0.02 ml |
| 10% AP | 0.05 ml | 0.02 ml |
| TEMED | 4 μl | 2 μl |