洗脱时间

A (乙腈)

B (0.1%甲酸水溶液)

0~10 min

15%

85%

10~15 min

15%~18%

85%~82%

15~20 min

18%

82%

20~40 min

18%~35%

82%~65%

40~42min

35%~45%

6%~55%

42~75 min

45%~85%

55%~15%