基因

引物序列

退火温度

预计产物长度

int1

int1F：GTTCGGTCAAGGTTCTGG

int1R：CGTAGAGACGTCGGAATG

55℃

890 bp

sul1

sul1F：GTGACGGTGTTCGGCATTCT

sul1R：TCCGAGAAGGTGATTGCGCT

66℃

779 bp

qacEΔ1

qacEΔ1F：ATCGCAATAGTTGGCGAAGT

qacEΔ1R：CAAGCTTTTGCCCATGAAGC

57℃

226 bp

int2

int2F：5'-CAAGCATCTCTAGGCGTA-3'

int2R：5'-AGTAGCATCAGTCAATCC-3'

55℃

1056 bp

int3

int3F：5'-CAAGCATCTCTAGGCGTA-3'

int3R：5'-AGTAGCATCAGTCAATCC-3'

55℃

878 bp