| 时间 Time (min) | 流速 Flow (mL/min) | 流动相A A (%) | 流动相B B (%) |
| 0.00 | 0.50 | 95.00 | 5.00 |
| 1.00 | 0.50 | 95.00 | 5.00 |
| 2.00 | 0.50 | 85.00 | 15.00 |
| 3.00 | 0.50 | 85.00 | 15.00 |
| 5.50 | 0.50 | 15.00 | 85.00 |
| 6.50 | 0.50 | 15.00 | 85.00 |
| 6.60 | 0.50 | 95.00 | 5.00 |
| 9.00 | 0.50 | 95.00 | 5.00 |