序号 | 靶序列所 在染色体 | 所在染色体的区域 | PCR产物大小(bp) | 与参考序列的 碱基差异数目(bp) | 引物F1错 配碱基数 | 引物R1错 配碱基数 | 引物结 合效率 |
1 | 1 | 16514122~16514285 | 164 | 0 | 0 | 0 | 72 |
2 | 1 | 16666785~16666948 | 164 | 0 | 0 | 0 | 72 |
3 | 1 | 16740516~16740679 | 164 | 0 | 0 | 0 | 72 |
4 | 1 | 16895980~16896143 | 164 | 0 | 0 | 0 | 72 |
5 | 1 | 143729407~143729570 | 164 | 0 | 0 | 0 | 72 |
6 | 1 | 146376807~146376970 | 164 | 0 | 0 | 0 | 72 |
7 | 1 | 144412576~144412740 | 165 | 18 | 2 | 0 | 56 |
8 | 1 | 144560666~144560829 | 164 | 1 | 0 | 0 | 72 |
9 | 1 | 145465617~145465780 | 164 | 2 | 0 | 0 | 72 |
10 | 1 | 148038753~148038916 | 164 | 1 | 0 | 1 | 72 |
11 | 1 | 148522601~148522765 | 165 | 15 | 0 | 1 | 53 |
12 | 4 | 135995929~135996092 | 164 | 5 | 0 | 1 | 72 |
13 | 7 | 141727984~141728148 | 165 | 13 | 1 | 1 | 72 |
14 | 14 | 34546714~34546877 | 164 | 0 | 0 | 0 | 72 |
15 | 14 | 34556226~34556389 | 164 | 0 | 0 | 0 | 72 |
16 | 15 | 44884616~44884781 | 166 | 20 | 2 | 2 | 72 |
17 | 15 | 45002863~45003028 | 166 | 18 | 2 | 2 | 72 |
18 | X | 119423742~119423905 | 164 | 8 | 2 | 2 | 72 |