| 试验号 | 试验方案 | 评价指标 | |||
| A 酶量(%) | B 酶解温度(℃) | C pH | D 酶解时间(h) | 吸光值(A) | |
| 1 | 1 (0.12) | 1 (35) | 1 (3.5) | 1 (4.5) | 0.266 ± 0.004 |
| 2 | 1 | 2 (40) | 2 (4.0) | 2 (5.0) | 0.284 ± 0.015 |
| 3 | 1 | 3 (45) | 3 (4.5) | 3 (5.5) | 0.282 ± 0.013 |
| 4 | 2 (0.15) | 1 | 2 | 3 | 0.274 ± 0.029 |
| 5 | 2 | 2 | 3 | 1 | 0.302 ± 0.004 |
| 6 | 2 | 3 | 1 | 2 | 0.274 ± 0.006 |
| 7 | 3 (0.18) | 1 | 3 | 2 | 0.283 ± 0.018 |
| 8 | 3 | 2 | 1 | 3 | 0.283 ± 0.002 |
| 9 | 3 | 3 | 2 | 1 | 0.298 ± 0.017 |
| K1 | 0.277 | 0.274 | 0.274 | 0.289 |
|
| K2 | 0.283 | 0.290 | 0.285 | 0.287 | |
| K3 | 0.288 | 0.285 | 0.289 | 0.280 | |
| R | 0.011 | 0.016 | 0.015 | 0.009 | |
| 因素主次 | DABC | ||||
| 最佳组合 | A1B1C1D3 | ||||