标记名称

标记及探针序列

badh2变异

位置

扩增产物

扩增及检测方法

育种应用

文献

Aromarker

第7外显子发生8bp缺失

400,392 bp

品种选育

[30]

BAD2

ESP: TTGTTTGGAGCTTGCTGATG IFAP: CATAGGAGCAGCTGAAATATATACC INSP: CTGGTAAAAAGATTATGGCTTCA EAP: AGTGCTTTACAAAGTCCCGC

第7外显子发生8bp缺失

577,585 bp 355 bp 257 bp

等位基因特异性PCR AGAR

资源鉴定

[32]

Bad2.7CAPS

Bad2.7Forward: CCAAGTGAAGGGGGTTGGCATTG Bad2.7Reverse: ACCAAAGGCATGATGTCAGGTCG

第14外显子1bp插入

255,188,121,67 bp, BslI

普通PCR AGAR

资源鉴定

[33]

BADEX7-5

F: TGTTTTCTGTTAGGTTGCATT R: ATCCACAGAAATTTGGAAAC

第7外显子发生8bp缺失

95,103 bp

普通PCR AGAR

资源鉴定

[34]

BADEX7-5

F: TGTTTTCTGTTAGGTTGCATT R: ATCCACAGAAATTTGGAAAC

第7外显子发生8bp缺失

95,103 bp

高分辨率溶解曲线(荧光标记)

资源鉴定

[35]

BADH2_KASP

Primer_AlleleFAM: TAACCATAGGAGCAGCTGAAG Primer_AlleleHEX: ACCTTAACCATAGGAGCAGCTGAAA Common reverse: TGCATTTACTGGGAGTTATGAAACTGGTA

第7外显子发生8bp缺失

KASP 荧光定量(荧光)

恢复系选育

[36]

badh2-p-5'UTR

F: ATAATATCGGTCGAAACATTTTTATT R: GAGAAGCTTTTACACTTTATGCACATC

5‘UTR的3bp和启动子8bp插入

480,158 &322 bp, AluI

普通PCR AGAR

资源鉴定

[37]

badh2-PCR/LDR

E2-F: GGGAGGCGCTGAAGAGGAAC E2-R: GTCACCACCCTACCTTGGC Probe-FLO: P-CGGCGCGCGCGCCCAGTCGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT-FAM Probe-E2: TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCGGAGGTACTTGGCCCGGA Probe-NON: TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGGTACTTGGCCCGGACGGCGCC

第2外显子的7 bp的缺失

111,109 nt

普通PCR ABI3730 (荧光)

资源鉴定

[31]

CAPS(Ehe^I)

F: AGGCGCTGAAGAGGAAC R: TAGTCACCACCCTACCTTG

第2外显子突变

292,375,667 bp, Ehe^I

普通PCR AGAR

资源鉴定

[38]