| 试验号 | 时间/min | 空列 | 温度/℃ | 空列 | 酶浓度/% | H2O2/mL | 试验方案 |
| 1 | 20 | 1 | 20 | 1 | 0 | 2.2 | A1B1C1D1 |
| 2 | 20 | 2 | 30 | 2 | 2.5 | 2.7 | A1B2C2D2 |
| 3 | 20 | 3 | 40 | 3 | 5 | 3.2 | A1B3C3D3 |
| 4 | 20 | 4 | 50 | 4 | 7.5 | 3.7 | A1B4C4D4 |
| 5 | 20 | 5 | 60 | 5 | 10 | 4.2 | A1B5C5D5 |
| 6 | 40 | 1 | 30 | 3 | 7.5 | 4.2 | A2B2C4D5 |
| 7 | 40 | 2 | 40 | 4 | 10 | 2.2 | A2B3C5D1 |
| 8 | 40 | 3 | 50 | 5 | 0 | 2.7 | A2B4C1D2 |
| 9 | 40 | 4 | 60 | 1 | 2.5 | 3.2 | A2B5C2D3 |
| 10 | 40 | 5 | 20 | 2 | 5 | 3.7 | A2B1C3D4 |
| 11 | 60 | 1 | 40 | 5 | 2.5 | 3.7 | A3B3C2D4 |
| 12 | 60 | 2 | 50 | 1 | 5 | 4.2 | A3B4C3D5 |
| 13 | 60 | 3 | 60 | 2 | 7.5 | 2.2 | A3B5C4D1 |
| 14 | 60 | 4 | 20 | 3 | 10 | 2.7 | A3B1C5D2 |
| 15 | 60 | 5 | 30 | 4 | 0 | 3.2 | A3B2C1D3 |
| 16 | 80 | 1 | 50 | 2 | 10 | 3.2 | A4B4C5D3 |
| 17 | 80 | 2 | 60 | 3 | 0 | 3.7 | A4B5C1D4 |
| 18 | 80 | 3 | 20 | 4 | 2.5 | 4.2 | A4B1C2D5 |
| 19 | 80 | 4 | 30 | 5 | 5 | 2.2 | A4B2C3D1 |
| 20 | 80 | 5 | 40 | 1 | 7.5 | 2.7 | A4B3C4D2 |
| 21 | 100 | 1 | 60 | 4 | 5 | 2.7 | A5B5C3D2 |
| 22 | 100 | 2 | 20 | 5 | 7.5 | 3.2 | A5B1C4D3 |
| 23 | 100 | 3 | 30 | 1 | 10 | 3.7 | A5B2C5D4 |
| 24 | 100 | 4 | 40 | 2 | 0 | 4.2 | A5B3C1D5 |
| 25 | 100 | 5 | 50 | 3 | 2.5 | 2.2 | A5B4C2D1 |