时间(min)

流动相(A)

流动相(B)

0~5

10%~20%

90%~80%

5~25

20%~30%

80%~70%

25~35

30%~35%

70%~65%

35~65

35%~70%

65%~30%

65~95

70%~85%

30%~15%

95~100

85%

15%