时间(min) | 流动相(A) | 流动相(B) |
0~5 | 10%~20% | 90%~80% |
5~25 | 20%~30% | 80%~70% |
25~35 | 30%~35% | 70%~65% |
35~65 | 35%~70% | 65%~30% |
65~95 | 70%~85% | 30%~15% |
95~100 | 85% | 15% |