| 管号 试剂 | 0.1 mg/mL葡萄糖标准体积(mL) | 蒸馏水体积(mL) | DNS体积(mL) | 葡萄糖质量(mg) | 吸光度A540 |
| 0 | 0 | 2 | 1.5 | 0 | 0 |
| 1 | 0.2 | 1.8 | 1.5 | 0.2 | 0.231 |
| 2 | 0.4 | 1.6 | 1.5 | 0.4 | 0.504 |
| 3 | 0.6 | 1.4 | 1.5 | 0.6 | 0.775 |
| 4 | 0.8 | 1.2 | 1.5 | 0.8 | 1.095 |
| 5 | 1.0 | 1.0 | 1.5 | 1.0 | 1.353 |