| 试剂名称 | 用量 |
| 基因组DNA | 40~60 ng |
| *Vn F (10 μM) | 1.5 μL |
| *Vn R (10 μM) | 1.5 μL |
| Q5 High-Fidelity DNA Polymerase | 0.2 μL |
| High GC Enhancer | 10 μL |
| Buffer | 10 μL |
| dNTP | 1 μL |
| ddH2O | 补至总体系50 µL |