引物名称 The name of primers | 序列 Sequences | 限制酶切位点 Restriction enzyme sites | 退火温度 |
P1937-F | atatggatccgtaacgtggcaaaacgaacaatgtctcactagactaaagtgagatcgacaaagcttaatt | BamHI、HindIII | 72.1℃ |
P1937-R | aattaagctttgtcgatctcactttagtctagtgagacattgttcgttttgcctcgttacggatccatat | BamHI、HindIII | 72.1℃ |
Ptac-M-F | atataagcttaattgttatccgctcacaattccacacattatacgagccgatgattaattgtcaacagctcaggatccaatt | BamHI、HindIII | 71.7℃ |
Ptac-M-R | aattggatcctgagctgttgacaattaatcatcggctcgtataatgtgtggaattgtgagcggataacaattaagcttatat | BamHI、HindIII | 71.7℃ |
16s rDNA-F | gtagggtgcgagcgttgtc | — | 57.6℃ |
16s rDNA-R | cgccattggtgttcctcctg | — | 55.9℃ |
cat-F | tcagctggatattacggcct | — | 51.8℃ |
cat-R | gaaagacggtgagctggtga | — | 53.8℃ |