| 时间(min) | 流速(μL∙min−1) | A(%) | B(%) |
| 0 | 400 | 90.0 | 10.0 |
| 2 | 400 | 90.0 | 10.0 |
| 9 | 400 | 5.0 | 95.0 |
| 16 | 400 | 5.0 | 95.0 |
| 18 | 400 | 90.0 | 10.0 |
| 20 | 400 | 90.0 | 10.0 |