| 试剂 试管号 | 1 | 2 | 3 | 4 | 5 | 6 |
| 标准蛋白质溶液(ml) | 0.0 | 0.2 | 0.4 | 0.6 | 0.8 | 1.0 |
| 蒸馏水(ml) | 1.0 | 0.8 | 0.6 | 0.4 | 0.2 | 0.0 |
| G-250试剂(ml) | 5.0 | 5.0 | 5.0 | 5.0 | 5.0 | 5.0 |
| 蛋白质含量(ug) | 0.0 | 20.0 | 40.0 | 60.0 | 80.0 | 100.0 |