DNAman (℃)

Oligo (℃)

IDT (℃)

NEB (℃)

Hf_Pf: 5’-CAGCCATATGAT_12-FamGAG T_16-BHQ1 G CC G-3’

62.6

56.4

56, S

56.1, D

56

66

Pr: 5’-CATATGGCTGCCGCGCGGCACC-3’

78.6

69

67.3

79