Primer

Sequence

Length (nt)

p16_170X sense

5'-GCAGCGCTCGAGTGGCGGAGCT-3'

22

p16_170X anti

5'-GCCACTCGAGCGCTGCCCATCAT-3'

23

p21_175X sense

5'-ACACCACTCGAGGGTGACTTCGCCT-3'

25

p21_175X anti

5'-ACCCTCGAGTGGTGTCTCGGTGA-3'

23

p27_210X sense

5'-ACCCCTCGAGGGCAAGTACGAGT-3'

23

p27_210X anti

5'-TGCCCTCGAGGGGTTTGTGATTCT-3'

24

CDK4_629E sense

5'-CGTCGAATTCCTCTCTTCTG-3'

19

CDK4_629E anti

5'-AGAGAGGAATTCGACGAAAC-3'

20

cyclin E_M2 sense

5'-TTTCGCTGCCGCGGCTGCGGCGACACAAGA AAATG-3'

35

cyclin E_M2 anti

5'-CGCCGCAGCCGCGGCAGCGAAATCTTGTGCCAA-3'

33

RB 1786X sense

5'-TCTTCCTCTCGAGAATAATCACACTG-3'

26

RB 1786X anti

5'-TGATTATTCTCGAGAGGAAGATTAAG-3'

26

T7 Promoter

5'-TAATACGACTCACTATAGG-3'

19

BGH

5'-TAGAAGGCACAGTCGAGG-3'

18

pEGFP_C'3

5'-TATGGCTGATTATGATCAGT-3'

20