| EcoRI Primer(+3)5'to3 | MseI Primer(+3)5'to3' | ||
| E-AAC | GACTGCGTACCAATTCAAC | M-CAA | GATGAGTCCTGAGTAACAA |
| E-ACC | GACTGCGTACCAATTCACC | M-CCA | GATGAGTCCTGAGTAACCA |
| E-ACA | GACTGCGTACCAATTCACA | M-CAG | GATGAGTCCTGAGTAACAG |
| E-AGG | GACTGCGTACCAATTCAGG | M-CGA | GATGAGTCCTGAGTAACGA |
| E-ACG | GACTGCGTACCAATTCACG | M-CTA | GATGAGTCCTGAGTAACTA |
| E-ACT | GACTGCGTACCAATTCACT | M-CAT | GATGAGTCCTGAGTAACAT |
| E-AGC | GACTGCGTACCAATTCAGC | M-CAC | GATGAGTCCTGAGTAACAC |