| 步骤 Step | 温度(℃) Temperature | 时间 Time |
| 1 | 95 | 5 min |
| 2 | 95 | 30 s |
| 3 | 58 | 30 s |
| 4 | 72 | 2 min 40 s |
| 5 | × 33 |
|
| 6 | 72 | 5 min |
| 7 | 4 | 10 min |