时间(min) | 0 | 10 | 23 | 28 | 33 | 41 | 46 | 50 | 55 | 60 |
流动相A (%) | 45 | 55 | 59 | 62 | 62 | 78 | 85 | 85 | 45 | 45 |
流动相B (%) | 55 | 45 | 41 | 38 | 38 | 22 | 15 | 15 | 55 | 55 |