| 引物名称 | 引物序列 |
| sgRNA2-T7fw | GAAATTAATACGACTCACTATAgtggagccagaggtcgctcaGTTTTAGAGCTAGAAATAGCAAG |
| T7rev common | AAAAGCACCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTTTAACTTGCTATTTCTAGCTCTA |
| CAS9-T7F | GAAATTAATACGACTCACTATAGGGAGAATGGACTATAAGGACCACGAC |
| CAS9-T7R | GCGAGCTCTAGGAATTCTTAC |
| Adcy3-mCherry-F | ctctgttacactgccccaccaagtggtggacaacccTATGGTGAGCAAGGGCGAGGA |
| Adcy3-mCherry-R | ccccttcctatgtggaccactgtggagccagaggtcTTACTTGTACAGCTCGTCCA |
| mCherry-HK13 F | CACGAGTTCGAGATCGAGGG |
| mCherry-HK14 R | GGTGTAGTCCTCGTTGTGGG |
| Adcy3-Outer-F | CCATGTACAGGTGGTAGAAGAG |
| Adcy3-Outer-R | TGGAATGTGCGCTTCAATAA |
| Adcy3-Inner-F | CCTTCGAGAGTACGGCTTCC |
| Adcy3-Inner-R | GGAACACCAGGACTTGGTCA |