| Reaction Component | Concentration | Volume (µl) |
| SybrGreen qPCR Master Mix | 2X | 10 |
| 引物F (10 uM) | 10 µM | 0.4 |
| 引物R (10 uM) | 10 µM | 0.4 |
| ddH20 | 7.2 | |
| Template (cDNA) | 2 | |
| Total |
| 20 µl |