Thermal Cycler | Times and Temperatures | |||
Initial Steps | Each of 45 cycles | |||
Melt | Anneal | Extend | ||
ABI Stepone plus型荧光定量PCR仪 | HOLD | CYCLE | ||
3 min 95℃ | 7 s 95℃ | 10 s 57℃ | 15 s 72℃ |